Complete, sensitive and fast viral genomics

with SMARTer® Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian

Sequencing the SARS-CoV-2 virus causing COVID-19 pandemics is crucial for public health authorities to understand identity and genomic changes of the virus and its transmission patterns.

Takara Bio supports researchers and clinicians studying this novel coronavirus with the SMARTer® Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian, which was used for SARS-CoV-2 virus sequencing.

Widely used solution for viral genomics

Thanks to built-in human ribosomal depletion and fast protocol, the SMARTer® Stranded kit has been widely used by world leading institutes for viral genomics and genomic epidemiology.

Virus
Sample type
Reference
SARS-CoV-2
Bronchoalveolar lavage fluid
Wu, F. et al., A new coronavirus associated with human respiratory disease in China. Nature. Mar;579(7798):265-269. (2020)
Metatranscriptomics - infectome
Throat swabs
Li CX. et al., High resolution metagenomic characterization of complex infectomes in paediatric acute respiratory infection. Sci Rep. Mar 3;10(1):3963. (2020)
HIV
Plasma
Bonsall D. et al., A comprehensive genomics solution for HIV surveillance and clinical monitoring in a global health setting. bioRxiv 2018.08.28.397083 (2018)
TBEV (Tick-borne encephalitis virus)
Infected cells
Cheval J. et al., Adventitious Virus Detection in Cells by High-Throughput Sequencing of Newly Synthesized RNAs: Unambiguous Differentiation of Cell Infection from Carryover of Viral Nucleic Acids. mSphere. Jun 5;4(3). pii: e00298-19. (2019)
MRV (Mammalian orthoreovirus)
Infected cells
Yamamoto S.P. et al., Novel human reovirus isolated from children and its long-term circulation with reassortments. Sci Rep. Jan 22;10(1):963. (2020)
Jingmenvirus
Serum
Temmam S. et al., Insights into the Host Range, Genetic Diversity, and Geographical Distribution of Jingmenviruses. mSphere. Nov 6;4(6). pii: e00645-19. (2019)
HBV (Hepatitis B virus)
CSF (cerebrospinal fluid)
Pettersson J.H. et al., Meta-transcriptomic identification of hepatitis B virus in cerebrospinal fluid in patients with central nervous system disease. Diagn Microbiol Infect Dis. Dec;95(4):114878. (2019)
HHV-4 (Epstein-Barr)
Infected cells
Brussel A. et al., Use of a new RNA next generation sequencing approach for the specific detection of virus infection in cells. Biologicals. May;59:29-36. (2019)

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