Webinar:
Cas9/sgRNA ribonucleoprotein (RNP) delivery to improve your KO and KI gene editing experiments

While CRISPR/Cas9 is a powerful technique for gene editing, two significant challenges remain: obtaining efficient delivery of Cas9 and sgRNA to a broad range of cell types, and leaving no additional footprint (i.e., persistent and elevated expression of Cas9 in target cells) that could lead to off-target effects.

Knockout mutations can usually be obtained with high efficiency, but knockin of longer sequences (more than 200 bp) via homology directed repair is more difficult to achieve. Although single-stranded DNA (ssDNA) donor templates have recently been shown to have several advantages over double-stranded DNA, the usefulness of long ssDNA templates is limited due to the difficulty and high cost of producing them. Takara Bio has recently developed a simple and economical method for generating long ssDNA donor templates up to 5 kb.

Join us for a webinar presented by Dr. Cornelia Hampe (Product Manager for Genome Editing at Takara Bio Europe) and learn about the benefits of Cas9/sgRNA RNP delivery. She will also explain the features of Takara Bio’s Guide-it Long ssDNA Production System and the benefits of using long ssDNA repair templates for knockin experiments.