Innovative tools to accelerate vaccine research and development

The recent emergence of the lethal coronavirus, SARS-CoV-2 (COVID-19), has led to an urgent need to rapidly develop a vaccine against this novel virus. Takara Bio offers an array of innovative technologies to support researchers and clinicians in their efforts to quickly develop a suitable vaccine, using reverse vaccinology workflows:

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Reverse vaccinology workflow

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1. Viral & host genomics

Whole-genome sequencing of the SARS-CoV-2 virus can provide valuable information on potential antigenic epitopes that could trigger an immune response. Indeed, Takara Bio's SMARTer® Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian has been used in China to sequence SARS-CoV-2, providing full-length viral genome sequence.

In addition, BCR and TCR immunoprofiling of COVID-19 infected or recovered individuals via RNA-seq can provide additional information to further fuel the process of antigen and vaccine target identification. Our highly sensitive SMARTer Human BCR and TCR profiling kits can be used to monitor these changes in the BCR-TCR clonotype repertoires of infected or recovered COVID-19 patients.

For automated, high-throughput, human TCR profiling at the single-cell level, SMARTer TCR kits have been adapted for the ICELL8® single-cell system. The appropriate sequencing data analysis pipeline was developed to allow the TCR profiling of single T-cells. Furthermore, it is possible to access the gene expression of the selected T-cell.


2. Bioinformatic analysis

Bioinformatic analysis to reveal SARS-CoV-2 antigens and selection of vaccine targets (viral surface receptors).


3. Cloning vaccine targets

Researchers can speed up viral receptor and vaccine construct generation, including HTP cloning, using our In-Fusion® HD Cloning technology:

  • Highly efficient—over 95% cloning efficiency for inserts ranging from 0.5 to 15 kb
  • Sequence independent—clone ANY insert, into ANY vector, at ANY locus
  • Seamless construction—no extra base pairs (or scar sequences) added to inserts
  • Versatile—multiple insert cloning and site directed mutagenesis in a single step

Indeed, a number of publications have already utilized In-Fusion® HD Cloning technology within their workflows to study and develop vaccines against coronaviruses, including SARS-CoV-2:


4. Expressing & purifying vaccine targets

Researchers can then rapidly purify His-tagged vaccine candidate proteins using our revolutionary Capturem high-capacity membrane-based purification technology:

  • Fast, convenient workflow—5 to 30 minute room-temperature protocol using convenient spin columns/plates/filtration devices featuring Capturem nickel-functionalized membranes
  • High purity—small bed volumes trap fewer contaminants
  • Compatible with wide range of additives—including EDTA, DTT, BME, glycerol, TCEP, etc.; see compatibility table)
  • Versatile—easy purification from mammalian/bacterial cell lysates and supernatants

Selected publications have already utilized our Capturem His technology within their vaccine development workflows:


5. Immunize mice with vaccine targets, screen serum for antiviral activity, & optimize promising candidates

Takara Bio's SMARTer Mouse BCR and TCR profiling Kits can be used to speed up the process of immune serum screening for antiviral activity of target vaccines. Promising vaccine candidates are then further optimized prior to going into clinical trials.


6. Clinical trials with selected final vaccine candidates

Monitoring the immune response is key to assessing the effectiveness of the final vaccine candidates. BCR and TCR repertoire profiling by NGS allows precise monitoring of clonotype changes and identification of vaccine-specific clonotypes.

Moreover, the ICELL8® single-cell system in conjunction with our adapted SMARTer TCR kit chemistry, can be utilized here to automate and perform high-throughput TCR profiling at the single-cell level.

Explore Takara Bio's products to accelerate vaccine research and development:

Cat # Products Size
638909 In-Fusion® HD Cloning Plus 10 Rxns
638910 In-Fusion® HD Cloning Plus 50 Rxns
638911 In-Fusion® HD Cloning Plus 100 Rxns
638920 In-Fusion® HD Cloning Plus 96 Rxns
639650 In-Fusion® HD Cloning Kit 100 rxns
638933 In-Fusion® HD Cloning Kit 500 Rxns
639691 In-Fusion® HD EcoDry™ Cloning Kit 96 rxns
635710 Capturem™ His-Tagged Purification Miniprep Kit 20 Rxns
635713 Capturem™ His-Tagged Purification Maxiprep Kit 6 Purifications
635714 Capturem™ His-Tagged Purification 96 96 Purifications
635730 Capturem™ His-Tagged Purification 24-Well Plate 1 Plate
635724 Capturem™ His-Tagged Purification Large Volume 4 Units
634466 SMARTer® Human BCR IgG IgM H/K/L Profiling Kit 12 Rxns
634467 SMARTer® Human BCR IgG IgM H/K/L Profiling Kit 48 Rxns
635014 SMARTer® Human TCR a/b Profiling Kit 12 Rxns
635015 SMARTer® Human TCR a/b Profiling Kit 48 Rxns
635016 SMARTer® Human TCR a/b Profiling Kit 96 Rxns
634431 SMARTer® Human scTCR a/b Profiling Kit 96 Rxns
634432 SMARTer® Human scTCR a/b Profiling Kit 480 Rxns
634422 SMARTer® Mouse BCR IgG H/K/L Profiling Kit 12 Rxns
634423 SMARTer® Mouse BCR IgG H/K/L Profiling Kit 48 Rxns
634424 SMARTer® Mouse BCR IgG H/K/L Profiling Kit 96 Rxns
634403 SMARTer® Mouse TCR a/b Profiling Kit 48 Rxns
634404 SMARTer® Mouse TCR a/b Profiling Kit 96 Rxns
640179 ICELL8® Human TCR a/b Profiling - Indexing Primer Set 1 Chip
640180 ICELL8® Human TCR a/b Profiling - Indexing Primer Set 5 Chips
640181 ICELL8® Human TCR a/b Profiling - Indexing Primer Set 10 Chips
640188 ICELL8® cx Single-Cell System Each

Contact us today to discuss your COVID-19 vaccine development projects

E-mail our Tech Support team at [email protected]. We'd happy to discuss your projects.

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