1. Viral & host genomics
Whole-genome sequencing of the SARS-CoV-2 virus can provide valuable information on potential antigenic epitopes that could trigger an immune response. Indeed, Takara Bio's SMARTer® Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian has been used in China to sequence SARS-CoV-2, providing full-length viral genome sequence.
In addition, BCR and TCR immunoprofiling of COVID-19 infected or recovered individuals via RNA-seq can provide additional information to further fuel the process of antigen and vaccine target identification. Our highly sensitive SMARTer Human BCR and TCR profiling kits can be used to monitor these changes in the BCR-TCR clonotype repertoires of infected or recovered COVID-19 patients.
For automated, high-throughput, human TCR profiling at the single-cell level, SMARTer TCR kits have been adapted for the ICELL8® single-cell system. The appropriate sequencing data analysis pipeline was developed to allow the TCR profiling of single T-cells. Furthermore, it is possible to access the gene expression of the selected T-cell.